A simple device to immobilize protists for electrophysiology and microinjection

by Anirudh Kulkarni, Irene Elices, Nicolas Escoubet, Léa-Laetitia Pontani, Alexis Michel Prevost, Romain Brette
Abstract:
We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.
Reference:
Anirudh Kulkarni, Irene Elices, Nicolas Escoubet, Léa-Laetitia Pontani, Alexis Michel Prevost, Romain Brette, 2020. A simple device to immobilize protists for electrophysiology and microinjection, Journal of Experimental Biology, volume 223.
Bibtex Entry:
@article{Kulkarni2020,
    author = {Kulkarni, Anirudh and Elices, Irene and Escoubet, Nicolas and Pontani, Léa-Laetitia and Prevost, Alexis Michel and Brette, Romain},
    title = "{A simple device to immobilize protists for electrophysiology and microinjection}",
    journal = {Journal of Experimental Biology},
    volume = {223},
    number = {12},
    year = {2020},
    month = {06},
    abstract = "{We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.}",
    issn = {0022-0949},
    doi = {10.1242/jeb.219253},
    url = {https://journals.biologists.com/jeb/article-pdf/223/12/jeb219253/1980525/jeb219253.pdf},
}