A simple device to immobilize protists for electrophysiology and microinjection

by Anirudh Kulkarni, Nicolas Escoubet, Léa-Laetitia Pontani, Alexis Michel Prevost, Romain Brette
Abstract:
We present a simple device to mechanically immobilize motile cells such as ciliates and flagellates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they immobilize against the filter. The cells swim again freely as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye in the cytosol.
Reference:
Anirudh Kulkarni, Nicolas Escoubet, Léa-Laetitia Pontani, Alexis Michel Prevost, Romain Brette, 2019. A simple device to immobilize protists for electrophysiology and microinjection, bioRxiv.
Bibtex Entry:
@article{Kulkarni2019,
	title = {A simple device to immobilize protists for electrophysiology and microinjection},
	url = {https://www.biorxiv.org/content/10.1101/859413v2.full.pdf},
	doi = {10.1101/859413},
	abstract = {We present a simple device to mechanically immobilize motile cells such as ciliates and flagellates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they immobilize against the filter. The cells swim again freely as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye in the cytosol.},
	journal = {bioRxiv},
	author = {Kulkarni, Anirudh and Escoubet, Nicolas and Pontani, Léa-Laetitia and Prevost, Alexis Michel and Brette, Romain},
	month = dec,
	year = {2019},
	pages = {859413}
}